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Nicholas Valerie - Barncancerfonden Swedish Childhood

Nikolai Zhelev 2017-06-01 · At 20 μM, the ATM inhibitor increased the Dox-evoked LDH release (Fig. 1E, right panel). In summary, these data show that the ATM inhibitor is protective in the Dox model of cell damage at concentrations of 1 μM and 1–10 μM in UN- and RA-SH-SY5Y cells, respectively, without clear exacerbation of cell damage at its higher concentrations. 3.4. The ATM inhibitor KU55933 sensitizes radioresistant bladder cancer cells with DAB2IP gene defect Int J Radiat Biol . 2015 Apr;91(4):368-78.

The ATM inhibitor KU55933 sensitizes radioresistant bladder cancer cells with DAB2IP gene defect Int J Radiat Biol . 2015 Apr;91(4):368-78. doi: 10.3109/09553002.2015.1001531. KU-55933 is a potent ATM inhibitor with an IC 50 and K i of 12.9 and 2.2 nM, respectively, and is highly selective for ATM as compared to DNA-PK, PI3K/PI4K, ATR and mTOR. IC 50 & Target [1] ATM Here we demonstrate that the treatment of intracellular tachyzoites with the PI3K inhibitor caffeine or ATM kinase-inhibitor KU-55933 affects parasite replication rate in a dose-dependent manner. KU-55933 affects intracellular tachyzoite growth and induces G1-phase arrest. In this study, we have observed that in contrast to the ATM selective inhibitor KU55933, ATM/ATR dual inhibitors such as caffeine and CGK733 can suppress cyclin D1 levels in cancer cell lines.

Upphävande av wip1-uttryck med rita-aktiverad p53 potentierar

In this study, we have observed that in contrast to the ATM selective inhibitor KU55933, ATM/ATR dual inhibitors such as caffeine and CGK733 can suppress cyclin D1 levels in cancer cell lines. A recent study suggests that mitogen-activated cyclin D1 expression is required for the induction of premature senescence . KU55933, which suppresses ATM phosphorylation upon irradiation, could be applied in the radiotherapy of BCa patients with a DAB2IP gene defect. Keywords:: DAB2IP , radiotresistance , bladder cancer , ATM , KU55933 2D chemical structure image of ab120637, KU-55933, competitive ATM kinase inhibitor.

Nicholas Valerie - Barncancerfonden Swedish Childhood

In summary, these data show that the ATM inhibitor is protective in the Dox model of cell damage at concentrations of 1 μM and 1–10 μM in UN- and RA-SH-SY5Y cells, respectively, without clear exacerbation of cell damage at its higher concentrations. 3.4. The ATM inhibitor KU55933 sensitizes radioresistant bladder cancer cells with DAB2IP gene defect Int J Radiat Biol . 2015 Apr;91(4):368-78. doi: 10.3109/09553002.2015.1001531.

KU55933 inhibits insulin- and IGF1-stimulated Akt phosphorylation at both Ser473 and Thr308. A ATM cellular inhibition by KU55933 was demonstrated in additional phosphorylation targets, including p53 Ser15, H2AX Ser139, NBS1 Ser343, Chk1 Ser345, and SMC1 Ser966. KU-55933 is a potent ATM inhibitor with an IC50 and K of 12.9 and 2.2 nM, respectively, and is highly selective for ATM as compared to DNA-PK, PI3K/PI4K, ATR and mTOR.
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Ataxia-telangiectasia mutated (ATM) kinase is a component of a signalling mechanism that determines the process of decision-making in response to DNA damage and involves the participation of multip KU55933 is a selective and reversible inhibitor of the activity of ATM and thus can be used to transiently inhibit ATM kinase activity in cells . Previous work suggested that ATM has temporally distinct functions in cells exposed to IR. KU-55933 (ATM Kinase Inhibitor) from Selleck Chemicals LLC. Product Specs; Item KU-55933 (ATM Kinase Inhibitor) Company Selleck Chemicals LLC; Catalog Number S1092; KU-55933 (ATM Kinase Inhibitor) KU-55933 (ATM Kinase Inhibitor) is a potent and specific ATM inhibitor with IC50 / Ki of 12.9 nM/2.2 nM in cell-free assays, and is highly selective for ATM as compared to DNA-PK, PI3K/PI4K, ATR and mTOR. 2009-11-10 · The components of the ATM and ATR regulated signaling pathways thus provide attractive pharmacological targets, since their inhibition enhances cellular sensitivity to chemo- and radiotherapy. Caffeine as well as more specific inhibitors of ATM (KU55933) or ATM and ATR (CGK733) have recently been shown to induce cell death in drug-induced senescent tumor cells.

To determine if pharmacological inactivation of ATM has similar effects as genetic inactivation, we used the ATM kinase inhibitor KU55933. Potent and selective ATM kinase inhibitor IC 50 s= 13, 2500, 9300, 16600, >100000 and >100000 nM for ATM, DNA-PK, mTOR, PI 3-kinase, PI 4-K and ATR respectively.
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Examining the Dynamics of Cellular Adhesion and Spreading

The established ATM inhibitor KU55933 was used as a positive control for ATM inhibition (24). IR-induced ATM kinase activity resulted in the expected increases in ATM-dependent phosphorylation events and CP466722 treatment inhibited all of these events. ATP-competitive ATM inhibitors like KU-55933 might act in a similar way as kd ATM protein, which, upon prolonged exposure, may cause greater side effects in vivo than a loss of ATM protein expression would. Further studies will need to address this question, but the possibility should be considered for future clinical development of ATM inhibitors.

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Arf utlöser senescens i brca2-bristfälliga celler genom att

Packaging 5, 25 mg in glass bottle Biochem/physiol Actions KU-55933 is a very potent, specific inhibitor of Ataxia telangiectasia (A-T) mutated (ATM) kinase (IC 50 = 13 nM). KU-22933 treatment sensitizes cancer cells to ionizing radiation and cytotoxic drugs. Functional Studies - KU-55933, competitive ATM kinase inhibitor (ab120637) HepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). As an ATM inhibitor, KU-55933 significantly inhibited the increase of phospho-Akt at Ser473 in MDA-MB-453 and PC-3 cells treated with insulin and IGF-I following serum starvation. In the MTT assay, KU-55933 treatment suppressed cell proliferation by about 50% at concentration of 10 μM in MDA-MB-453 and PC-3 cells.

Cellcykelberoende hämning av 53bp1 signalering med brca1

ATM deficiency increases TMZ sensitivity, which suggests that ATM inhibitors may be effective TMZ sensitizing agents. In this study, the TMZ sensitizing effects of 2 ATM specific inhibitors were studied in established and xenograft 2012-11-21 · KU-55933 is a specific inhibitor of the kinase activity of the protein encoded by Ataxia telangiectasia mutated (ATM), an important tumor suppressor gene with key roles in DNA repair. Unexpectedly for an inhibitor of a tumor suppressor gene, KU-55933 reduces proliferation. In view of prior preliminary evidence suggesting defective mitochondrial function in cells of patients with Ataxia Pharmacological ATM inhibition by KU55933 in cisplatin-treated mice did not ameliorate, but instead exacerbated cisplatin-induced DNA damage and tubular injury, thereby increasing mortality.

KU55933 (KU)-induced ATM upregulation is accompanied by an increase in pATM and E2F1 concentrations.